Render Target: STATIC
Render Timestamp: 2024-10-09T09:42:01.085Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ALAD (E8Z7Y) Rabbit mAb #76798

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 36
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ALAD (E8Z7Y) Rabbit mAb recognizes endogenous levels of total ALAD protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human ALAD protein.

    Background

    Delta-aminolevulinic acid dehydratase (ALAD) catalyzes the second step in heme biosynthesis. ALAD is also an endogenous inhibitor of the 26S proteasome (1). Defects in ALAD cause acute hepatic porphyria, an inherited defect in heme biosynthesis in the liver (2). ALAD activity is inhibited by lead, and defects in ALAD result in sensitivity to lead poisoning (3). Reduced expression of ALAD is associated with poor prognosis in breast cancer patients. Overexpression of ALAD in breast cancer cells negatively regulates proliferation, invasion, and transforming growth factor-β (TGF-β)-mediated epithelial-mesenchymal transition (EMT) (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.