Alix (3A9) Mouse mAb #2171
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Alix (3A9) Mouse mAb detects endogenous levels of total Alix protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length recombinant human Alix protein.
Background
Alix, a phylogenetically conserved cytosolic scaffold protein, contains an N-terminal Bro1 domain, a coiled-coil region and a C-terminal proline-rich domain (1,2). Originally identified as an ALG-2 (apoptosis-linked gene 2)-interacting protein involved in programmed cell death (3,4), Alix also regulates many other cellular processes, such as endocytic membrane trafficking and cell adhesion through interactions with ESCRT (endosomal sorting complex required for transport) proteins, endophilins, and CIN85 (Cbl-interacting protein of 85 kDa) (5,6).
- Mattei, S. et al. (2006) Eur. J. Cell Biol. 85, 925-936.
- Odorizzi, G. (2006) J. Cell Sci. 119, 3025-3032.
- Vito, P. et al. (1999) J. Biol. Chem. 274, 1533-1540.
- Missotten, M. et al. (1999) Cell Death Differ. 6, 124-129.
- Katoh, K. et al. (2003) J. Biol. Chem. 278, 39104-39113.
- Sadoul, R. (2006) Biol. Cell 98, 69-77.
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