ALOX15 (E5D1C) Rabbit mAb #82129
- WB
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:500 - 1:1000 |
Storage
Protocol
Specificity / Sensitivity
ALOX15 (E5D1C) Rabbit mAb recognizes endogenous levels of total ALOX15 protein. This antibody does not cross-react with ALOX15B protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to a region of human ALOX15 protein.
Background
Polyunsaturated fatty acid lipoxygenase ALOX15, also known as arachidonate 15-lipoxygenase (15-LOX, 15-LOX-1), catalyzes oxygenation of polyunsaturated fatty acids to corresponding hydroperoxides (1,2). Studies show that the small scaffolding protein PEBP1 associates with ALOX15 and polyunsaturated fatty acid lipoxygenase ALOX15B to change their substrate specificity, resulting in the conversion of polyunsaturated phosphatidylethanolamines (PE) to hydroperoxy-PE. Hydroperoxy-PE, when not sufficiently reduced by GPX4, causes ferroptosis (3). In addition, a loss-of-function mutation in ALOX15 leads to protection against nasal polyps and chronic rhinosinusitis (4). Furthermore, ALOX15-positive macrophages contribute to the pathogenesis of a subtype of chronic rhinosinusitis (5).
Limited Uses
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