Render Target: STATIC
Render Timestamp: 2025-03-19T10:41:50.799Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:19:53.780
Product last modified at: 2025-03-07T08:00:56.934Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ALPP/ALPPL2 (F1L4G) Rabbit mAb #30971

Filter:
  • WB
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunohistochemistry (Paraffin) 1:1000 - 1:4000
    Immunofluorescence (Immunocytochemistry) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ALPP/ALPPL2 (F1L4G) Rabbit mAb recognizes endogenous levels of total ALPP and ALPPL2 protein. This antibody does not cross-react with ALPI or ALPL protein but it may cross-react with a protein of unknown origin at 25 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ALPP protein.

    Background

    Mammalian alkaline phosphatases (ALPs) are highly conserved zinc-containing allosteric enzymes that hydrolyze and transphosphorylate a wide range of compounds (1). There are four known human alkaline phosphatase isozymes: ALPL (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPPL2 (germ cell), and ALPI (intestinal) (2). Placental alkaline phosphatase (ALPP) is bound to the plasma membrane via a glycosyl-phosphatidylinositol (GPI) anchor (3). It is expressed primarily in the placenta (4) and may be involved in transplacental IgG transport (5). ALPP has been found to be overexpressed on the surface of several different types of solid tumor cells (6), and elevated serum concentrations of ALPP and ALPP-like enzymes has been found to be associated with ovarian, cervical, and testicular cancer (7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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