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ALPPL2 (F9B1D) Rabbit mAb #45883

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  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:1600 - 1:6400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ALPPL2 (F9B1D) Rabbit mAb recognizes endogenous levels of total ALPPL2 protein. This antibody does not cross-react with other ALP proteins. This antibody detects a 140 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala89 of human ALPPL2 protein.

    Background

    Mammalian alkaline phosphatases (ALPs) are highly conserved zinc-containing allosteric enzymes that hydrolyze and transphosphorylate a wide range of compounds (1). There are four known human alkaline phosphatase isozymes: ALPL (tissue-nonspecific; bone/liver/kidney), ALPP (placental), ALPPL2 (germ cell), and ALPI (intestinal) (2). Alkaline phosphatase placental-like 2 (ALPPL2) shares high (98%) homology with alkaline phosphatase placental (ALPP), but is uniquely expressed in the thymus, testis, and certain germ cell cancers such as seminoma (3-6). ALPPL2 has become an attractive immunotherapy target due to its restricted expression in healthy tissue and overexpression in various cancers (7). In addition to seminoma, elevated ALPPL2 expression has been identified in gastric and pancreatic cancers and mesothelioma (7-9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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