Androgen Receptor Antibody Sampler Kit #61949
Product Information
Kit Usage Information
Protocols
- 5153: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow, ChIP Magnetic, Chromatin IP-seq, CUT&RUN Assay
- 7074: Western Blotting
- 19672: Western Blotting, Immunoprecipitation (Magnetic), ChIP Magnetic
- 70317: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence
Product Description
Specificity / Sensitivity
Each antibody in the Androgen Receptor Antibody Sampler kit detects endogenous levels of its target protein. Androgen Receptor (D6F11) XP® Rabbit mAb detects both full-length AR and the AR-V7 isoform. Androgen Receptor (E3S4N) Rabbit mAb (Carboxy-terminal Antigen) detects only full-length AR. Androgen Receptor (AR-V7 Specific) (E3O8L) Rabbit mAb only detects the AR-V7 isoform.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with recombinant protein corresponding to residues near the amino terminal region of human androgen receptor protein, and with synthetic peptides corresponding to residues surrounding Val662 of human androgen receptor protein and Leu639 of human androgen receptor (V7 isoform) protein.
Background
Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).
The AR3 or AR-V7 isoform, which lacks the typical ligand binding domain, is created through the alternative splicing of cryptic exons (4-5). AR-V7 is frequently expressed in castration-resistant prostate cancer (CRPC) and while dependent on the activity of the full-length androgen receptor (AR-FL), AR-V7 can activate a completely distinct transcriptional program (6-8). While enzalutamide and abiraterone have been beneficial in treating CRPC through the ligand binding domain of AR-FL, resistance in patients has been shown to be associated with AR-V7 detection in circulating tumor cells (9-12). Studies probing into mechanisms of overcoming this resistance are currently being explored and may help in stratifying patient populations for more personalized therapies (13-15).
- Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8.
- Avila, D.M. et al. J Steroid Biochem Mol Biol 76, 135-42.
- Montgomery, J.S. et al. (2001) J Pathol 195, 138-46.
- Hu, R. et al. (2009) Cancer Res 69, 16-22.
- Guo, Z. et al. (2009) Cancer Res 69, 2305-13.
- Watson, P.A. et al. (2010) Proc Natl Acad Sci U S A 107, 16759-65.
- Sun, S. et al. (2010) J Clin Invest 120, 2715-30.
- Hu, R. et al. (2012) Cancer Res 72, 3457-62.
- Scher, H.I. et al. (2012) N Engl J Med 367, 1187-97.
- de Bono, J.S. et al. (2011) N Engl J Med 364, 1995-2005.
- Ryan, C.J. et al. (2013) N Engl J Med 368, 138-48.
- Antonarakis, E.S. et al. (2014) N Engl J Med 371, 1028-38.
- Liu, C. et al. (2014) Clin Cancer Res 20, 3198-3210.
- Sarwar, M. et al. (2016) Oncotarget 7, 63065-63081.
- Ku, S.Y. et al. (2017) Science 355, 78-83.
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