AP3B1 (E7U2P) Rabbit mAb #95765
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 135 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
AP3B1 (E7U2P) Rabbit mAb recognizes endogenous levels of total AP3B1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human AP3B1 protein.
Background
The adaptor protein complex 3 (AP-3) is a ubiquitously expressed, heterotetramer that is comprised of two large adaptins (AP3D1 and either AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2), and a small adaptin (AP3S1 or AP3S2). The AP-3 complex is involved in the sorting of protein cargo within the endosomal compartment that are destined for lysosomes (1,2). Defects in the AP-3 complex have been associated with Hermansky-Pudlak syndrome (HPS), a genetic disorder characterized by abnormal skin pigmentation, blood clotting abnormalities, and pulmonary fibrosis. Mutations in the gene encoding ubiquitously expressed AP3B1/β3A, destabilize the protein and the entire AP-3 complex, which results in defective trafficking of lysosomal membrane proteins (3,4). Research studies have shown that certain viral proteins have evolved to co-opt the AP-3 complex by binding to AP3B1 in order to facilitate viral particle assembly (5).
Limited Uses
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