Render Target: STATIC
Render Timestamp: 2024-10-11T09:51:17.405Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-10 22:03:16.930
Product last modified at: 2024-09-19T08:00:17.185Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ApoE2 (E6U9I) Rabbit mAb (BSA and Azide Free) #75018

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Recombinant protein
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #62273. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #62273

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    ApoE2 (E6U9I) Rabbit mAb (BSA and Azide Free) recognizes overexpressed levels of total ApoE2 protein. This antibody does not cross-react with ApoE3 or ApoE4 by western blot. This antibody may detect non-specific bands of unknown origin at 55 kDa and 70 kDa.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys176 of human ApoE2 protein.

    Background

    Apolipoproteins are plasma lipoproteins that function as transporters of lipids and cholesterol in the circulatory system. Chylomicrons are a fundamental class of apolipoproteins containing very low-density lipoproteins (VLDL), intermediate-density lipoproteins (IDL), low-density lipoproteins (LDL), and high-density lipoproteins (HDL) (1,2).

    Human ApoE has three isoforms: ApoE2, ApoE3, and ApoE4. These three isoforms differ in the combination of cysteine and arginine residues located at positions 130 and 176. The ApoE2 isoform contains cysteine at both locations. ApoE4 has been identified as a genetic risk factor for Alzheimer’s disease (AD), where ApoE2 is neuroprotective, reducing risk of AD by nearly 50%. The neuroprotective mechanism of ApoE2 remains under investigation, but studies suggest that this may be due to its overall protein abundance in non-AD human cortex and its unique lipid binding profile compared to ApoE3 and ApoE4, which may increase overall β-amyloid clearance (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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