Render Target: STATIC
Render Timestamp: 2024-10-14T10:37:41.905Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:59:26.206
Product last modified at: 2024-09-30T08:00:56.216Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

AQP4 (E4B6T) Mouse mAb #74806

Filter:
  • WB
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Mouse IgG2b kappa
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Frozen) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    AQP4 (E4B6T) Mouse mAb recognizes endogenous levels of total AQP4 protein. This antibody detects a 55 kDa band of unknown origin.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AQP4 protein.

    Background

    Aquaporins (AQP) are integral membrane proteins that serve as channels in the transfer of water and small solutes across the membrane. There are 13 isoforms of AQP that express in different types of cells and tissues (1,2). AQP1 is found in blood vessels, kidney, eye, and ear. AQP2 is found in the kidney, and it has been shown that the lack of AQP2 results in diabetes (1,3). AQP4 is present in the brain, where it is enriched in astrocytes (1,2,4). AQP5 is found in the salivary and lacrimal gland, AQP6 in intracellular vesicles in the kidney, AQP7 in adipocytes, AQP8 in kidney, testis, and liver, AQP9 is present in liver and leukocytes, and AQP10-11 in the intestine (1,3,4). AQPs are essential for the function of cells and organs. It has been shown that AQP1 and AQP4 regulate the water homeostasis in astrocytes, preventing cerebral edema caused by solute imbalance (5). Several studies have shown the involvement of AQPs in the development of inflammatory processes, including cells of innate and adaptive immunity (6,7).

    AQP4 is expressed in brain cells, including neurons, but enriched in astrocytes, as well as in the peripheral nervous system (4,7). AQP4 influences synaptic plasticity, and the lack of this protein in the brain may cause memory and learning impairment via glutamate transporter 1 (GLT1) in Alzheimer's disease (8-10). It's suggested that AQP4 could play a role in the clearance of β-amyloid, and it may influence the transport of potassium and calcium in Alzheimer's disease (8,9). In Parkinson's disease, the lack of AQP4 results in an increased susceptibility of neurons from the substantia nigra and the ventral tegmental area for MPTP (11).
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