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89872
Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

Arginase-1 (D4E3M) XP® Rabbit mAb (BSA and Azide Free) #89872

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from mouse liver and mouse small intestine using Arginase-1 (D4E3M) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from mouse and rat liver using Arginase-1 (D4E3M) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human liver using Arginase-1 (D4E3M) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Arginase-1 (D4E3M) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Arginase-1 (D4E3M) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse liver using Arginase-1 (D4E3M) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse liver (left) or small intestine (right) using Arginase-1 (D4E3M) XP® Rabbit mAb (green) and DRAQ5 #4084 (fluorescent DNA dye) (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of mouse primary bone marrow-derived macrophages (BMDMs) using Arginase-1 (D4E3M) XP® Rabbit mAb (green). BMDMs were differentiated with M-CSF (20 ng/ml, 7 days) and activated with either IL-4/cAMP (20 ng/ml, 0.5 mM, 24 hours; left) or LPS/IFNγ (50 ng/ml, 20 ng/ml, 24 hours; right). Red = Propidium Iodide (PI)/RNase Staining Solution #4087. Data were generated using the standard formulation of this product.
To Purchase # 89872SF
Product # Size Price
89872SF
100 µg $ 746

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 40
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #93668. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #93668

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Arginase-1 (D4E3M™) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total arginase-1 protein. This antibody does not cross-react with arginase-2.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val47 of human arginase-1 protein.

Background

L-arginine plays a critical role in regulating the immune system (1-3). In inflammation, cancer and certain other pathological conditions, myeloid cell differentiation is inhibited leading to a heterogeneous population of immature myeloid cells, known as myeloid-derived suppressor cells (MDSCs). MDSCs are recruited to sites of cancer-associated inflammation and express high levels of arginase-1 (4). Arginase-1 catalyzes the final step of the urea cycle converting L-arginine to L-ornithine and urea (5). Thus MDSCs increase the catabolism of L-arginine resulting in L-arginine depletion in the inflammatory microenvironment of cancer (4,6). The reduced availability of L-arginine suppresses T-cell proliferation and function and thus contributes to tumor progression (4,6). Arginase-1 is of great interest to researchers looking for a therapeutic target to inhibit the function of MDSCs in the context of cancer immunotherapy (7). In addition, research studies have demonstrated that Arginase-1 distinguishes primary hepatocellular carcinoma (HCC) from metastatic tumors in the liver, indicating its value as a potential biomarker in the diagnosis of HCC (8,9).
  1. Albina, J.E. et al. (1989) J Exp Med 169, 1021-9.
  2. Mills, C.D. (2001) Crit Rev Immunol 21, 399-425.
  3. Rodriguez, P.C. et al. (2004) Cancer Res 64, 5839-49.
  4. Gabrilovich, D.I. and Nagaraj, S. (2009) Nat Rev Immunol 9, 162-74.
  5. Wu, G. and Morris, S.M. (1998) Biochem J 336 ( Pt 1), 1-17.
  6. Raber, P. et al. (2012) Immunol Invest 41, 614-34.
  7. Wesolowski, R. et al. (2013) J Immunother Cancer 1, 10.
  8. Sang, W. et al. (2015) Tumour Biol 36, 3881-6.
  9. Geramizadeh, B. and Seirfar, N. (2015) Hepat Mon 15, e30336.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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