Render Target: STATIC
Render Timestamp: 2025-03-20T10:56:59.449Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:20:29.702
Product last modified at: 2025-02-25T20:45:08.560Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. ARID5A (F7T9F) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ARID5A (F7T9F) Rabbit mAb #35393

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 75
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ARID5A (F7T9F) Rabbit mAb recognizes endogenous levels of total ARID5A protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu154 of human ARID5A protein.

    Background

    AT-rich interactive domain-containing protein 5A (ARID5A) is a member of the ARID family, which includes 15 distinct human proteins. ARID is a nucleic acid binding domain that has a helix-turn-helix motif and is conserved in all eukaryotes (1,2). ARID5A expression is seen in many immune cells, including macrophages, CD4+ T cell subsets TH1 and TH17, as well as stromal cells and keratinocytes (2). During inflammation, ARID5A is translocated from the nucleus to the cytoplasm and stabilizes a variety of mRNA transcripts, including IL-6, Stat3, OX40, T-bet, and IL-17, contributing to the inflammatory response and a variety of inflammatory diseases. TLR4-activated NF-kB and MAPK pathways are involved in regulating ARID5A expression (3). ARID5A plays an important role in resistance to chimeric antigen receptor (CAR)-T cell therapy by stabilizing IDO1 mRNA and the upregulation of IDO1 expression. Elevated IDO1 expression levels facilitate the conversion of tryptophan to kynurenine, which contributes to CAR-T cell exhaustion (4).

    Database Links

    UniProt ID: Q03989


    Entrez-Gene Id: 10865


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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