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  3. AsCpf1/Cas12a (Strain BV3L6) (3D3-F7) Mouse mAb (BSA and Azide Free)

AsCpf1/Cas12a (Strain BV3L6) (3D3-F7) Mouse mAb (BSA and Azide Free) #53555

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    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa) 151
    Source/Isotype Mouse IgG2a
    Application Key:
    • WB-Western Blotting 
    • ELISA-ELISA 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #93300. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #93300

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    AsCpf1/Cas12a (Strain BV3L6) (3D3-F7) Mouse mAb (BSA and Azide Free) recognizes transfected levels of total AsCpf1/Cas12a (Strain BV3L6) protein. This antibody does not cross-react with Cas9 (S. pyogenes), Cas9 (S. aureus), or FnCpf1/Cas12a (Strain U112) proteins.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of Acidaminococcus sp. Cpf1/Cas12a (Strain BV3L6) protein.

    Background

    CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from Prevotella and Francisella) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a endonucleases, compared to Cas9 systems, have several unique features that increase the utility of CRISPR-based genome editing techniques: 1) Cpf1/Cas12a-mediated cleavage relies on a single and short CRISPR RNA (crRNA) without the requirement of a trans-activating crRNA (tracrRNA), 2) Cpf1/Cas12a utilizes T-Rich protospacer-adjacent motif (PAM) sequences rather than a G-Rich PAM, and 3) Cpf1/Cas12a generates a staggered, rather than a blunt-ended, DNA double-stranded break (2). These features broaden the utility of using CRISPR-Cas systems for specific gene regulation and therapeutic applications. Several Cpf1/Cas12a bacterial orthologs have been characterized for CRISPR-mediated mammalian genome editing (2,4).
    AsCpf1 (Strain BV3L6)/Cas12a is a Cpf1/Cas12a enzyme derived from Acidaminococcus sp. BV3L6 (5,6).

    Database Links

    UniProt ID: U2UMQ6


    Entrez-Gene Id:


    Limited Uses

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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