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69974
ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free) #69974

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  1. WB
  2. IHC
  3. IF
  4. F
Western Blotting Image 1: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from HT-29 cells, untreated or treated with peptide N-glycosidase F (PNGase F), and untreated SW620 cells using ASCT2 (D7C12) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using ASCT2 (D7C12) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using ASCT2 (D7C12) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using ASCT2 (D7C12) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ASCT2 (D7C12) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded SW480 cell pellet (left, high-expressing) or SK-MEL-5 cell pellet (right, low-expressing) using ASCT2 (D7C12) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using ASCT2 (D7C12) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using ASCT2 (D7C12) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HT-29 cells using ASCT2 (D7C12) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free)
Flow cytometric analysis of fixed and permeabilized HT-29 cells using ASCT2 (D7C12) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Data were generated using the standard formulation of this product.
To Purchase # 69974
Cat. # Size Qty. Price
69974SF
100 µg
$ 758

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 49, 75
Source/Isotype Rabbit 

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #8057. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #8057

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

ASCT2 (D7C12) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total ASCT2 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ASCT2 protein.

Background

Sodium-dependent neutral amino acid transporter type 2 (ASCT2 or SLC1A5) is a neutral amino acid transporter that regulates the uptake of essential amino acids in conjunction with the SLC7A5 bilateral transporter (1,2). ASCT2 appears to be the major glutamine transporter in hepatoma cells and is thought to provide essential amino acids needed for tumor growth (3). Additional evidence suggests that ASCT2 plays a role in activating mTORC1 signaling and is required to suppress autophagy (4,5). Cell surface ASCT2 serves as a receptor for several mammalian interference retroviruses associated with cases of infectious immunodeficiency; variation in a small region of an extracellular loop (ECL2) may be responsible for species-specific differences in receptor function (6).
  1. Utsunomiya-Tate, N. et al. (1996) J Biol Chem 271, 14883-90.
  2. Bröer, S. (2008) Physiol Rev 88, 249-86.
  3. Bode, B.P. et al. (2002) Am J Physiol Gastrointest Liver Physiol 283, G1062-73.
  4. Fuchs, B.C. et al. (2007) Am J Physiol Cell Physiol 293, C55-63.
  5. Nicklin, P. et al. (2009) Cell 136, 521-34.
  6. Marin, M. et al. (2003) J Virol 77, 2936-45.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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