Render Target: STATIC
Render Timestamp: 2024-10-21T10:09:56.888Z
Commit: 4d55b15f5e0754a7adc0cf65b75f728b5d0d4ec3
XML generation date: 2024-09-20 06:15:40.142
Product last modified at: 2024-10-18T11:45:10.623Z
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

ATGL Antibody #2138

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 54
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ATGL Antibody detects endogenous levels of total ATGL protein.

    Species Reactivity:

    Human, Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence around Pro186 of human ATGL. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues (1-3). Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific (4). ATGL was independently identified as desnutrin (5) and the TG-hydrolace inducible phospholipase-A2-ζ (6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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