| Cat. # | Size | Qty. | Price |
|---|---|---|---|
| 46486T | 1 Kit (6 x 20 microliters) |
|
$ 518 |
| Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
|---|---|---|---|---|---|
| ULK1 (D8H5) Rabbit mAb 8054 | 20 µl |
|
H M R Mk | 150 | Rabbit IgG |
| Atg13 (D4P1K) Rabbit mAb 13273 | 20 µl |
|
H M R | 72 | Rabbit IgG |
| FIP200 (D10D11) Rabbit mAb 12436 | 20 µl |
|
H M | 200 | Rabbit IgG |
| Atg101 (E1Z4W) Rabbit mAb 13492 | 20 µl |
|
H M R Mk | 25 | Rabbit IgG |
| Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb 14202 | 20 µl |
|
H M R Mk | 140-150 | Rabbit IgG |
| Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb 5869 | 20 µl |
|
H M | 140-150 | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Rab | Goat |
Product Information
Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg600 of human ULK1 protein, residues surrounding Asp462 of human Atg13 protein, residues surrounding Val177 of human Atg101 protein, or residues near the carboxy terminus of human FIP200 protein. Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser555 of mouse ULK1 protein (equivalent to Ser556 of human ULK1) or residues surrounding Ser757 of mouse ULK1 protein (equivalent to Ser758 of human ULK1).
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. ULK1, Atg13, and FIP200 form a complex that localizes to autophagic isolation membranes and regulates autophagosome biogenesis (4-6). mTOR phosphorylates both Atg13 and ULK1, suppressing ULK1 kinase activity and autophagy (5-7). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (8,9). AMPK, activated during low nutrient conditions, directly phosphorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777 (7,10). Conversely, mTOR, which is a regulator of cell growth and is an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK (7).
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STRING - Known and Predicted Protein-Protein Interactions.
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