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46486T 1 Kit (6 x 20 µl) $409.00.0


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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
ULK1 (D8H5) Rabbit mAb 8054 20 µl
Western Blotting Immunoprecipitation
H M R Mk 150 Rabbit IgG
Atg13 (D4P1K) Rabbit mAb 13273 20 µl
Western Blotting Immunoprecipitation
H M R 72 Rabbit IgG
FIP200 (D10D11) Rabbit mAb 12436 20 µl
Western Blotting Immunoprecipitation
H M 200 Rabbit IgG
Atg101 (E1Z4W) Rabbit mAb 13492 20 µl
Western Blotting
H M R Mk 25 Rabbit IgG
Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb 14202 20 µl
Western Blotting Immunoprecipitation
H M R 140-150 Rabbit IgG
Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb 5869 20 µl
Western Blotting Immunoprecipitation
H M 140-150 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Autophagy Induction (ULK1 Complex) Antibody Sampler Kit provides an economical means of detecting target proteins in the ULK1 complex. The kit contains enough antibody to perform at least two western blot experiments per primary antibody.

Specificity / Sensitivity

ULK1 (D8H5) Rabbit mAb, Atg13 (D4P1K) Rabbit mAb, FIP200 (D10D11) Rabbit mAb, and Atg101 (E1Z4W) Rabbit mAb recognize total endogenous levels of the corresponding target proteins irrespective of phosphorylation state. Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb detects endogenous levels of ULK1 only when phosphorylated at Ser555 of mouse ULK1 (equivalent to Ser556 of human ULK1). Bands of unknown origin are detected between 90 and 100 kDa. Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb recognizes endogenous levels of ULK1 protein only when phosphorylated at Ser757 of mouse ULK1 (equivalent to Ser758 of human ULK1).

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg600 of human ULK1 protein, residues surrounding Asp462 of human Atg13 protein, residues surrounding Val177 of human Atg101 protein, or residues near the carboxy terminus of human FIP200 protein. Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser555 of mouse ULK1 protein (equivalent to Ser556 of human ULK1) or residues surrounding Ser757 of mouse ULK1 protein (equivalent to Ser758 of human ULK1).

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. ULK1, Atg13, and FIP200 form a complex that localizes to autophagic isolation membranes and regulates autophagosome biogenesis (4-6). mTOR phosphorylates both Atg13 and ULK1, suppressing ULK1 kinase activity and autophagy (5-7). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (8,9). AMPK, activated during low nutrient conditions, directly phosphorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777 (7,10). Conversely, mTOR, which is a regulator of cell growth and is an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK (7).

1.  Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.

2.  Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.

3.  Mercer, C.A. et al. (2009) Autophagy 5, 649-62.

4.  Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.

5.  Hosokawa, N. et al. (2009) Autophagy 5, 973-9.

6.  Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.

7.  Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

8.  Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.

9.  Kim, J. et al. (2011) Nat Cell Biol 13, 132-41.

10.  Egan, D.F. et al. (2011) Science 331, 456-61.

Entrez-Gene Id 60673 , 9776 , 9821 , 8408
Swiss-Prot Acc. Q9BSB4 , O75143 , Q8TDY2 , O75385

For Research Use Only. Not For Use In Diagnostic Procedures.
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Autophagy Induction (ULK1 Complex) Antibody Sampler Kit