Cell Signaling Technology Logo
1% for the planet logo
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

β-crystallin B1/crybb1 (F6Y9L) Rabbit mAb #60327

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    β-crystallin B1/crybb1 (F6Y9L) Rabbit mAb recognizes endogenous levels of total β-crystallin B1 protein. Non-specific nuclear staining was observed in many normal mouse tissues by immunohistochemistry.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse β-crystallin B1 protein.

    Background

    β-crystallin B1, encoded by crybb1, is a member of the β-crystallins, one of three major crystallin types encoded by the mammalian genome, which also includes alpha- and gamma-crystallins. Crystallins are expressed at very high levels in the retinal lens and form homo/heterodimers via its N-terminal arms (1). Crystallins generally function to maintain the transparency and refractive power of the eye lens. However, they are expressed outside of the lens in other tissues and have alternative non-lens functions (2). For example, Nuc1 mutant rat, in which the βA3/A-crystallin gene is mutated, exhibit vascular remodeling abnormalities via an astrocyte-dependent mechanism (3,4). The precise cellular function of β-crystallins in glia is not well understood but may involve regulation of endolysosomal acidification, Golgi trafficking, and specific forms of cell death (5,6). Interestingly, crybb1 is highly enriched in microglia outside of the mammalian lens and may serve as a distinct marker for microglia (7). 
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.