BAF (E4O8R) Rabbit mAb #98783
- WB
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 10,11 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
BAF (E4O8R) Rabbit mAb recognizes endogenous levels of total BAF protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala12 of human BAF protein.
Background
Barrier-to-autointegration factor (BAF or BANF1) plays a major role in the lifecycle of retroviruses, where it inhibits the reverse transcribed viral genome from integrating into itself (1-3). BAF exists as a dimer in solution, and serves as a bridge for double-stranded DNA in an ordered nucleoprotein complex (4). BAF interacts with LEM-domains of lamin binding proteins emerin and LAP2, and serves to help reform the nuclear envelope during telophase (5-7). The binding of BAF to its various partners is inhibited by Ser4 phosphorylation, which is deposited by vaccinia-related kinases (VRKs) and occurs during interphase and mitosis (8,9). Certain viral genomes encode kinases extremely similar to VRK to prevent BAF from binding to their DNA, impeding viral replication (10,11). BAF has been implicated in various tumor types and is associated with poor prognosis (12-14).
- Lee, M.S. and Craigie, R. (1994) Proc Natl Acad Sci USA 91, 9823-7.
- Lee, M.S. and Craigie, R. (1998) Proc Natl Acad Sci USA 95, 1528-33.
- Cai, M. et al. (1998) Nat Struct Biol 5, 903-9.
- Zheng, R. et al. (2000) Proc Natl Acad Sci USA 97, 8997-9002.
- Shumaker, D.K. et al. (2001) EMBO J 20, 1754-64.
- Lee, K.K. et al. (2001) J Cell Sci 114, 4567-73.
- Haraguchi, T. et al. (2001) J Cell Sci 114, 4575-85.
- Bengtsson, L. and Wilson, K.L. (2006) Mol Biol Cell 17, 1154-63.
- Nichols, R.J. et al. (2006) Mol Biol Cell 17, 2451-64.
- Wiebe, M.S. and Traktman, P. (2007) Cell Host Microbe 1, 187-97.
- Jamin, A. et al. (2014) PLoS One 9, e100511.
- Li, J. et al. (2017) Biomed Pharmacother 89, 1086-1091.
- Shen, Q. and Nam, S.W. (2018) BMB Rep 51, 57-58.
- Li, J. et al. (2018) Oncol Lett 16, 6488-6494.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.