Render Target: STATIC
Render Timestamp: 2024-12-12T10:56:15.238Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-08-30 10:37:22.622
Product last modified at: 2024-08-31T07:01:05.459Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

BATF3 (E3K5H) Rabbit mAb (BSA and Azide Free) #62132

Filter:
  • WB
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 18
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #16484. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #16484

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    BATF3 (E3K5H) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total human BATF3 protein. This clone exhibits reactivity by western blot in cell lines transfected with a construct expressing Myc/DDK-tagged mouse BATF3 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human BATF3 protein.

    Background

    BATF3 (Basic leucine zipper transcriptional factor ATF-like 3, p21SNFT) is a member of the basic leucine zipper family (1,2,5) which dimerizes with Jun proteins and represses activator protein-1 (AP-1) activity (1). BATF3 is required for development of conventional type 1 dendritic cells (cDC1s), which cross-present antigens in the context of MHC class I to naïve CD8+ T cells, priming them to become activated cytotoxic CD8+ T cells (2-4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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