Render Target: STATIC
Render Timestamp: 2024-12-10T11:27:31.713Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-19 16:02:16.500
Product last modified at: 2024-11-21T17:45:09.561Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Bax (2D2) Mouse mAb #89477

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 20
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 36 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.

    Protocol

    Specificity / Sensitivity

    Bax (2D2) Mouse mAb recognizes endogenous levels of total Bax protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the N-terminal region of human Bax protein.

    Background

    Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9, and initiation of the caspase activation pathway for apoptosis (3,4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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