Render Target: STATIC
Render Timestamp: 2024-11-11T11:02:47.898Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:16:19.751
Product last modified at: 2024-08-20T12:15:33.362Z
1% for the planet logo
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Bit1 Antibody #3543

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 19, 21
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Bit1 Antibody detects endogenous levels of total human Bit1 protein. It also detects overexpressed bovine Bit1 and may detect overexpressed levels of mouse and rat Bit1.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within a central region of Bit1. Antibodies were purified by protein A and peptide affinity chromatography.

    Background

    Bit1 is a mitochondrial protein discovered as a regulator of anoikis, a process of apoptosis triggered by the loss of cell adhesion to the extracellular matrix (1,2). Like several other mitochondrial apoptotic regulators (Cytochrome c, Smac, HtrA2, AIF, EndoG), Bit1 is released into the cytoplasm during apoptosis (1,3). Cytoplasmic Bit1 associates with the Groucho family of transcriptional regulator AES (1,4). Interaction between Bit1 and AES promotes apoptosis, and this complex is suppressed by integrin mediated cell attachment.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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