BMPR2 (E9U5C) Rabbit mAb #24803
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 150 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
BMPR2 (E9U5C) Rabbit mAb recognizes endogenous levels of total BMPR2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly853 of human BMPR2 protein.
Background
BMPR2 is a type II serine/threonine receptor kinase that binds to an array of secreted bone morphogenetic proteins (BMPs). BMPs belong to the superfamily of TGF-β ligands that modulate gastrulation, neurogenesis, chondrogenesis, interdigital cell death, and bone morphogenesis (1-5). In contrast to the TGF-β type II receptor, BMPR2 contains an extended carboxyl-terminal region that interacts with multiple signaling molecules to modulate the responsiveness of target genes to BMPs (6,7). BMP signaling requires oligomerization of both type I and type II receptors to elicit a functional response of target genes. BMP binding to type I and II receptors induces Smad1/5/8 phosphorylation which is required for the activation of target genes (7). In vitro and in vivo evidence suggests that defects in BMPR2 may contribute to pulmonary hypertension, inflammation, and endothelial injury (8,9).
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- Storm, E.E. and Kingsley, D.M. (1996) Development 122, 3969-79.
- Reddi, H. (1995) Adv Dent Res 9, 13.
- Zhang, H. and Bradley, A. (1996) Development 122, 2977-86.
- Dunn, N.R. et al. (1997) Dev Biol 188, 235-47.
- Kudo, T.A. et al. (2010) Tohoku J Exp Med 222, 121-9.
- Gilboa, L. et al. (2000) Mol Biol Cell 11, 1023-35.
- Machado, R.D. et al. (2001) Am J Hum Genet 68, 92-102.
- Long, L. et al. (2009) Circulation 119, 566-76.
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