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BRD3 (E3D5N) Rabbit mAb
Primary Antibodies
Monoclonal Antibody

BRD3 (E3D5N) Rabbit mAb #94032

Citations (0)
  1. WB
  2. IP
  3. ChIP
Western blot analysis of extracts from various cell lines using BRD3 (E3D5N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Low expression of BRD3 protein in NCI-H929 cells is consistent with the predicted expression pattern.
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or human BRD3 siRNA (hBRD3 siRNA; +), using BRD3 (E3D5N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Immunoprecipitation of BRD3 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BRD3 (E3D5N) Rabbit mAb. Western blot analysis was performed using BRD3 (E3D5N) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.

Chromatin immunoprecipitations were performed with cross-linked chromatin from MV-4-11 cells and either BRD3 (E3D5N) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human c-Myc promoter primers, human BCL2 promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

To Purchase # 94032
Cat. # Size Qty. Price
100 µl

Supporting Data

MW (kDa) 100
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&T-CUT&Tag
  • DB-Dot Blot
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Chromatin IP 1:50


Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

BRD3 (E3D5N) Rabbit mAb recognizes endogenous levels of total BRD3 protein.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala10 of human BRD3 protein.


Bromodomain-containing protein 3 (BRD3), also known as ORFX, is a member of the bromodomains and extra terminal (BET) family of proteins, which also includes BRD2, BRD4, and BRDT (1-3). BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues (3). BRD3 is capable of binding acetylated histone H3 Lys14 as well as acetylated histone H4 Lys5 and Lys12 to promote transcription (4). BRD3 plays a role in erythroid development by binding to GATA1 to facilitate its binding to target genes (5). Similar to BRD4, the BRD3 gene can be fused to NUT in NUT midline carcinomas (6). Investigators have found molecular inhibition of BET proteins to be effective in inducing apoptosis in various MLL-fusion driven leukemic cell lines by competing BRD3 and BRD4 from chromatin, leading to reduced expression of BCL2, Myc, and CDK6 (7).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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