c-Myc/N-Myc (D3N8F) Rabbit Monoclonal Antibody #13987
- WB
- IF
- F
- ChIP
- C&R
- C&T
Product Specifications
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 57-65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
- C&R-CUT & RUN
- C&T-CUT & Tag
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.
The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
| CUT&RUN | 1:50 |
| CUT&Tag | 1:50 |
Storage
For a carrier free (BSA and azide free) version of this product see product #83277.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Alternate Names
avian myelocytomatosis viral oncogene homolog; BHLHE37; BHLHE39; c-Myc; Class E basic helix-loop-helix protein 37; Class E basic helix-loop-helix protein 39; MODED; MRTL; MYC; Myc proto-oncogene protein; MYC proto-oncogene, bHLH transcription factor; myc-related translation/localization regulatory factor; MYCC; MYCN; MYCN proto-oncogene, bHLH transcription factor; N-myc; N-myc proto-oncogene protein; neuroblastoma MYC oncogene; neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene; NMYC; ODED; oncogene NMYC; pp65/67; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog; v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived; v-myc myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian); v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Limited Uses
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