CART (D6L8J) Rabbit mAb #14547
- IP
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 15 |
| Source/Isotype | Rabbit IgG |
Application Key:
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:100 |
Storage
Protocol
Specificity / Sensitivity
CART (D6L8J) Rabbit mAb recognizes endogenous levels of total CART protein. Based on IP data and western blot results using lysates from cells overexpressing CART, this antibody appears to be specific to the precursor form of CART.
Species Reactivity:
Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human CART protein.
Background
Cocaine- and amphetamine-regulated transcript (CART) peptides are neurotransmitters of 39 and 47 amino acids that are involved in a variety of physiological processes. The CART precursor, a polypeptide of 116 residues, requires prohormone/proprotein convertase-mediated endoproteolytic cleavage to produce the two active peptides (1). CART peptides are found in several neuroendocrine tissues such as the brain, pituitary, adrenals, and pancreas (2). Hypothalamic CART is regulated by leptin, and plays a role in appetite and feeding behavior (3). Mesolimbic CART is regulated by CREB and may play a role in drug abuse behaviors by mediating some of CREB effects (4). Pancreatic CART is found in islet endocrine cells and parasympathetic and sensory nerves. It inhibits glucose-stimulated insulin secretion and has been found to be up-regulated in beta cells in animal model of diabetes (5). A missense mutation in the corresponding CART gene can correlate with susceptibility to obesity and reduced resting energy expenditure (6).
- Stein, J. et al. (2006) Peptides 27, 1919-25.
- Hunter, R.G. and Kuhar, M.J. (2003) Curr Drug Targets CNS Neurol Disord 2, 201-5.
- Thim, L. et al. (1998) Int J Biochem Cell Biol 30, 1281-4.
- Kuhar, M.J. et al. (2005) AAPS J 7, E259-65.
- Wierup, N. and Sundler, F. (2006) Peptides 27, 2031-6.
- del Giudice, E.M. et al. (2001) Diabetes 50, 2157-60.
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