Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Cathepsin A (F8X9E) Rabbit Monoclonal Antibody #67452

Filter:
  • WB
  • IHC

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 54, 20
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cathepsin A (F8X9E) Rabbit Monoclonal Antibody recognizes endogenous levels of total cathepsin A protein. This antibody detects the full-length (inactive) cathepsin A precursor in addition to the processed (active) light chain. This antibody detects a 75 kDa protein of unknown identity in some cell lines by western blotting. Non-specific nuclear staining was observed in testis by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human cathepsin A protein.

    Background

    Cathepsin A, also known as lysosomal protective protein for beta-galactosidase (PPGB), functions as both a serine protease and a critical enzyme for stabilizing β-galactosidase and neuraminidase (1,2). Similar to other cathepsin family members, cathepsin A is synthesized as an inactive 54 kDa precursor, then cleaved into catalytically active large (32 kDa) and small (20 kDa) subunits that form a disulfide-linked heterodimer after excision of a linker peptide (1-3). Cathepsin A degradation targets include endothelin (4), LAMP2 (5), and angiotensin I (6). CTSA gene mutations result in protein misfolding and lysosomal galactosialidosis, as neuraminidase and β-galactosidase activity depend on direct association with cathepsin A (7,8). Cathepsin A overexpression in heart causes excess degradation of superoxide dismutase (SOD), increasing oxidative stress, inflammation, and remodeling of the extracellular matrix (ECM) (9). High expression of cathepsin A has been reported and associated with metastasis in melanocytic tumors, colorectal cancer, hepatocellular carcinoma, and lung adenocarcinoma (10-13).

    Alternate Names

    beta-galactosidase 2; beta-galactosidase protective protein; Carboxypeptidase C; Carboxypeptidase L; carboxypeptidase Y-like kininase; carboxypeptidase-L; Cathepsin A; CTSA; deamidase; GLB2; GSL; lysosomal carboxypeptidase A; Lysosomal protective protein; Lysosomal protective protein 20 kDa chain; Lysosomal protective protein 32 kDa chain; NGBE; PPCA; PPGB; Protective protein cathepsin A; Protective protein for beta-galactosidase; urinary kininase

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.