Cathepsin D (E5V4H) Rabbit mAb #74089
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 46, 43, 28 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunohistochemistry (Paraffin) | 1:75 - 1:300 |
| Immunofluorescence (Frozen) | 1:800 - 1:1600 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
Cathepsin D (E5V4H) Rabbit mAb detects endogenous levels of preprocathepsin D, procathepsin D, and the heavy chain subunit of mature cathepsin D protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu179 of mouse cathepsin D protein.
Background
Cathepsin D is a ubiquitously expressed lysosomal aspartyl protease involved in the normal degradation of proteins (1). It is synthesized as an inactive 43 kDa preprocathepsin D that is cleaved and glycosylated to form a 46 kDa procathepsin D and then further cleaved to produce 28 kDa and 15 kDa subunits (heavy and light chains, respectively) (2). Cathepsin D may also be secreted into the cytosol during apoptosis and contribute to cleavage of substrates implicated in the apoptotic pathway (3). Numerous studies have suggested that cathepsin D plays a role in neuronal degradation and malignant transformation, particularly in breast cancer (4-9).
- Faust, P.L. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 4910-4.
- Erickson, A.H. et al. (1981) J. Biol .Chem. 256, 11224-31.
- Liaudet-Coopman, E. et al. (2006) Cancer Lett. 237, 167-79.
- Berchem, G. et al. (2002) Oncogene 21, 5951-5.
- Nomura, T. and Katunuma, N. (2005) J. Med. Invest. 52, 1-9.
- Garcia, M. et al. (1996) Stem Cells 14, 642-50.
- Nogami, M. et al. (2000) Histochem. J. 32, 505-8.
- Nakanishi, H. (2003) Ageing Res. Rev. 2, 367-81.
- Callahan, L.M. et al. (1998) Neurobiol. Aging 19, S99-105.
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