Render Target: STATIC
Render Timestamp: 2024-10-11T09:38:26.091Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-08-30 10:37:06.999
Product last modified at: 2024-08-31T07:01:47.502Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Cathepsin K (E7U5N) Rabbit mAb (BSA and Azide Free) #89623

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 29, 43
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #57056. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #57056

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    Cathepsin K (E7U5N) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total cathepsin K protein. Non-specific focal cytoplasmic staining was observed in mouse testis by immunohistochemistry.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly230 of mouse cathepsin K protein.

    Background

    Cathepsin K (CTSK) is a cysteine proteinase belonging to the cathepsin family, which all share a common papain-like structural fold and a conserved active site Cys-Asn-His triad of residues (1-3). Cathepsin K, initially synthesized as an inactive procathepsin K, is further processed into a mature enzyme, whose most studied function is to mediate bone resorption (1-6). Originally discovered in osteoclasts, its expression has also been associated with various cancers, such as prostate, breast, bone, lung, and renal carcinoma (6). Many pharmaceutical companies are working on the development of selective inhibitors for cathepsin K for anti-osteoporosis drugs (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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