Caveolin-1 (D46G3) XP® Rabbit mAb (BSA and Azide Free) #44989
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R Hm Mk B Dg |
| SENSITIVITY | Endogenous |
| MW (kDa) | 21, 24 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
- Mk-Monkey
- B-Bovine
- Dg-Dog
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Caveolin-1 (D46G3) XP® Rabbit mAb (BSA and Azide Free) detects endogenous levels of total caveolin-1 protein.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey, Bovine, Dog
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu20 of human caveolin-1.
Background
The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2, and -3) have been identified with different tissue distributions. Caveolins form hetero- and homo-oligomers that interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion, and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases, and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).
- Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22.
- Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304.
- Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
- Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
- Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75.
- Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408.
Limited Uses
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