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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. CBX7 (E7N1W) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CBX7 (E7N1W) Rabbit mAb #34547

Filter:
  • WB
  • IHC
  • ChIP

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 35
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • ChIP-Chromatin Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:400 - 1:1600
    Chromatin IP 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CBX7 (E7N1W) Rabbit mAb recognizes endogenous levels of total CBX7 protein.


    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CBX7 protein.

    Background

    The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). Chromobox protein homolog 7 (CBX7) is a component of the PRC1 complex that binds to the H3K27me3 mark through its N-terminal chromodomain to facilitate transcriptional repression (7). CBX7 is believed to promote stem cell self-renewal via repression of differentiation-specific genes, in contrast to other CBX proteins that promote lineage commitment (8-10). CBX7 also plays a complex role in cancer, being described as either a tumor suppressor or proto-oncogene, depending on the cellular context and tissue type (7). For instance, CBX7 has been reported as an oncogene in follicular lymphoma and gastric cancer through repression of the INK4A/ARF tumor suppressor locus (11,12). In contrast, CBX7 functions as a tumor suppressor by negatively regulating CCNE1 expression in human lung carcinoma and glioma (13,14). Decreased CBX7 levels have also been correlated with increased malignancy and poor prognosis in other cancer types, including thyroid, bladder, pancreatic, breast, and colon carcinomas, suggesting a potential use for CBX7 as a prognostic biomarker (15-20).

    1. Boyer, L.A. et al. (2006) Nature 441, 349-53.
    2. Lee, T.I. et al. (2006) Cell 125, 301-13.
    3. Park, I.K. et al. (2003) Nature 423, 302-5.
    4. Molofsky, A.V. et al. (2003) Nature 425, 962-7.
    5. Tavares, L. et al. (2012) Cell 148, 664-78.
    6. Gao, Z. et al. (2012) Mol Cell 45, 344-56.
    7. Li, J. et al. (2021) Front Genet 12, 740794.
    8. Morey, L. et al. (2012) Cell Stem Cell 10, 47-62.
    9. Klauke, K. et al. (2013) Nat Cell Biol 15, 353-62.
    10. Geng, Z. and Gao, Z. (2020) Int J Mol Sci 21, 8594. doi: 10.3390/ijms21228594.
    11. Scott, C.L. et al. (2007) Proc Natl Acad Sci U S A 104, 5389-94.
    12. Zhang, X.W. et al. (2010) J Exp Clin Cancer Res 29, 114.
    13. Forzati, F. et al. (2012) J Clin Invest 122, 612-23.
    14. Yu, T. et al. (2017) Oncotarget 8, 26637-26647.
    15. Pallante, P. et al. (2008) Cancer Res 68, 6770-8.
    16. Hinz, S. et al. (2008) Tumour Biol 29, 323-9.
    17. Karamitopoulou, E. et al. (2010) Eur J Cancer 46, 1438-44.
    18. Mansueto, G. et al. (2010) Genes Cancer 1, 210-24.
    19. Pallante, P. et al. (2010) Eur J Cancer 46, 2304-13.
    20. Yang, Y. et al. (2021) Onco Targets Ther 14, 5477-5492.

    Database Links

    UniProt ID: O95931


    Entrez-Gene Id: 23492


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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