CCL20 (E6J9K) Rabbit mAb #76924
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 9 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
CCL20 (E6J9K) Rabbit mAb recognizes endogenous levels of total CCL20 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human CCL20 protein.
Background
Chemokine (C-C motif) ligand 20 (CCL20), also known as macrophage inflammatory protein (MIP)-3α, liver activation regulated chemokine (LARC), and Exodus-1, is a chemokine that mediates leukocyte migration during inflammation and homeostasis (1-3). CCL20 is expressed by T helper (Th) 17 cells (Th17), B cells, natural killer cells, neutrophils, dendritic cells (DC), macrophages, and endothelial cells (4-6). Its receptor, C-C chemokine receptor 6 (CCR6), is expressed by immune cells, such as regulatory CD4+ T cells (Tregs), Th17 cells, CD8+ T cells, and B cells, and drives their migration into CCL20 rich tissues (7). In induced Tregs, CCL20 ligation with its receptor suppresses Treg function via signaling through the AKT/mTOR/STAT3 axis (8). The CCR6-CCL20 axis is being investigated as a therapeutic target for its role in autoimmune disease, viral infection, and cancer progression (7,9).
- Power, C.A. et al. (1997) J Exp Med 186, 825-35.
- Hromas, R. et al. (1997) Blood 89, 3315-22.
- Kadomoto, S. et al. (2020) Int J Mol Sci 21, 5186. doi: 10.3390/ijms21155186.
- Yamazaki, T. et al. (2008) J Immunol 181, 8391-401.
- Scapini, P. et al. (2001) Eur J Immunol 31, 1981-8.
- Nandi, B. et al. (2014) PLoS One 9, e97566.
- Meitei, H.T. et al. (2021) Autoimmun Rev 20, 102846.
- Kulkarni, N. et al. (2018) J Autoimmun 88, 121-130.
- Chen, W. et al. (2020) Adv Exp Med Biol 1231, 53-65.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.