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  3. CCN3 (F1I3E) Rabbit Monoclonal Antibody
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CCN3 (F1I3E) Rabbit Monoclonal Antibody #80948

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  • WB

    Product Specifications

    REACTIVITY H R
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CCN3 (F1I3E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CCN3 protein. This antibody detects a 75 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CCN3 protein.

    Background

    Cellular communication network factor 3 (CCN3), also known as nephroblastoma-overexpressed (NOV), belongs to the CCN (Cyr61, Ctgf, NOV) family of proteins. It is a cystine-rich secretory protein that associates with components of the extracellular matrix. Like other CCN family members, CCN3 is capable of mediating diverse biological functions through its four distinct domains, which enable binding to numerous protein partners (1-5).

    CCN3 modulates bone turnover through various mechanisms and is implicated in the progression of primary bone cancers, such as osteosarcoma and chondrosarcoma (6-8). Research has shown that CCN3 is also involved in the bone metastasis of melanoma, breast cancer, and prostate cancers (9-11). Recently, CCN3 was reported to play an important role in stem cell renewal (12). CCN3 is normally expressed in both embryonic and adult tissues (13,14). The activity of CCN3 is influenced by post-translational modifications and proteolytic cleavage (15,16).
    1. Perbal, B. (2001) Mol Pathol 54, 57-79.
    2. Brigstock, D.R. et al. (2003) Mol Pathol 56, 127-8.
    3. Leask, A. and Abraham, D.J. (2006) J Cell Sci 119, 4803-10.
    4. Yeger, H. and Perbal, B. (2007) J Cell Commun Signal 1, 159-64.
    5. McCallum, L. and Irvine, A.E. (2009) Blood Rev 23, 79-85.
    6. Perbal, B. et al. (2008) Clin Cancer Res 14, 701-9.
    7. Tzeng, H.E. et al. (2011) J Cell Physiol 226, 3181-9.
    8. Yang, W. et al. (2011) Hum Reprod 26, 2850-60.
    9. Vallacchi, V. et al. (2008) Cancer Res 68, 715-23.
    10. Ouellet, V. et al. (2011) Am J Pathol 178, 2377-88.
    11. Chen, P.C. et al. (2012) Carcinogenesis 33, 937-45.
    12. Gupta, R. et al. (2007) Science 316, 590-3.
    13. Burren, C.P. et al. (1999) J Clin Endocrinol Metab 84, 1096-103.
    14. Kocialkowski, S. et al. (2001) Anat Embryol (Berl) 203, 417-27.
    15. Perbal, B. et al. (1999) Proc Natl Acad Sci U S A 96, 869-74.
    16. Su, B.Y. et al. (2001) Mol Pathol 54, 184-91.

    Alternate Names

    CCN family member 3; CCN3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; IGFBP-9; IGFBP9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma overexpressed; nephroblastoma overexpressed gene; Nephroblastoma-overexpressed gene protein homolog; NOV; NOVH; Protein NOV homolog

    Database Links

    UniProt ID: P48745


    Entrez-Gene Id: 4856


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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