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67330
CD10/Neprilysin (F4P6H) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

CD10/Neprilysin (F4P6H) Rabbit mAb (BSA and Azide Free) #67330

Citations (0)
Filter:
  1. WB
  2. IHC
  3. IF
  4. F
Western blot analysis of extracts from various cell lines and tissues using CD10/Neprilysin (F4P6H) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of CD10/Neprilysin protein in NIH/3T3 and MBT2 cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CD10/Neprilysin (F4P6H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded MC-38 syngeneic tumor using CD10/Neprilysin (F4P6H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse lung using CD10/Neprilysin (F4P6H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse testis using CD10/Neprilysin (F4P6H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD10/Neprilysin (F4P6H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse oviduct using CD10/Neprilysin (F4P6H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse thymus using CD10/Neprilysin (F4P6H) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse small intestine using CD10/Neprilysin (F4P6H) Rabbit mAb (left) or a CD10/Neprilysin Rabbit mAb (right). These two antibodies detect unique, non-overlapping epitopes on CD10/Neprilysin. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded mouse liver using CD10/Neprilysin (F4P6H) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemical analysis of paraffin-embedded Renca cell pellet (left, positive) or MBT2 cell pellet (right, negative) using CD10/Neprilysin (F4P6H) Rabbit mAb. Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of fixed frozen mouse kidney labeled with CD10/Neprilysin (F4P6H) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 555 Conjugate) #19581 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of fixed frozen mouse liver labeled with CD10/Neprilysin (F4P6H) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with CD45 (D3F8Q) Rabbit mAb (Alexa Fluor® 555 Conjugate) #19581 (right, red), and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue). Data were generated using the standard formulation of this product.
Confocal immunofluorescent analysis of mIMCD-3 cells (left, positive) and NIH/3T3 cells (right, negative) using CD10/Neprilysin (F4P6H) Rabbit mAb (green), β-Actin (8H10D10) Mouse mAb #3700 (red), and DAPI #4083 (blue). Data were generated using the standard formulation of this product.
Flow cytometric analysis of fixed/permeabilized NIH/3T3 cells (blue, negative) and Renca cells (green, positive) using CD10/Neprilysin (F4P6H) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
To Purchase # 67330
Cat. # Size Qty. Price
67330SF
100 µg

Supporting Data

REACTIVITY M R
SENSITIVITY Endogenous
MW (kDa) 100
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #58145. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #58145

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD10/Neprilysin (F4P6H) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD10/Neprilysin protein.

Species Reactivity:

Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu493 of mouse CD10/Neprilysin protein.

Background

CD10, also known as Neprilysin, common acute lymphoblastic leukemia antigen (CALLA), or neutral endopeptidase (NEP), is a zinc-dependent transmembrane metallo-endopeptidase. CD10 cleaves peptides at the N-terminal side of hydrophobic amino acid residues and deactivates a variety of signaling peptides (1,2). CD10 was shown to be one of the markers for a natural killer cell-restricted progenitor in fetal and adult tissues (3). Studies also suggest CD10 as a phenotypic marker to distinguish mature neutrophils from immature neutrophils in patients with inflammation (2). In addition, CD10+ GPR77+ carcinoma-associated fibroblasts (CAFs) promote tumor formation and chemoresistance (4).

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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