CD13/APN (E1Y7U) Rabbit mAb #13721
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140-160 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
CD13/APN (E1Y7U) Rabbit mAb recognizes endogenous levels of total CD13/APN protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg90 of human CD13/APN protein.
Background
Aminopeptidase N (APN, CD13) is a widely expressed, membrane-bound proteolytic enzyme that breaks down peptides during digestion, cleaves cell surface antigens during antigen presentation, and acts as a receptor for human viruses, including several coronaviruses. This multifunctional protein is implicated in the regulation of many biological processes, including angiogenesis, cell proliferation, cell migration, inflammation and immune response (1,2). APN was originally identified as the cell surface antigen CD13, which is expressed in myeloid lineage hematopoietic cells and myeloid leukemia (3). Identified substrates of aminopeptidase N include the angiotensin I-III peptide hormones, the opioid peptide met-enkephalin, and cytokines MCP-1 and MIP-1 (4). Abnormal APN protein expression is seen in various forms of cancer, with high APN expression associated with poor survival in colon cancer and non-small cell lung cancer and silenced APN expression related to poor prognosis in prostate cancer (5-7).
- Luan, Y. and Xu, W. (2007) Curr Med Chem 14, 639-47.
- Mina-Osorio, P. (2008) Trends Mol Med 14, 361-71.
- Look, A.T. et al. (1989) J Clin Invest 83, 1299-307.
- Bauvois, B. (2004) Oncogene 23, 317-329.
- Hashida, H. et al. (2002) Gastroenterology 122, 376-86.
- Tokuhara, T. et al. (2006) Clin Cancer Res 12, 3971-8.
- Sørensen, K.D. et al. (2013) Br J Cancer 108, 420-8.
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