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CD13 (B-F10) Mouse Monoclonal Antibody #3566

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Inquiry Info. # 3566

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 150
    Source/Isotype Mouse IgG1
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:25

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD13 (B-F10) Mouse Monoclonal Antibody detects endogenous levels of CD13.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing BALB/c mice with KG-1 cells.

    Background

    Aminopeptidase N (APN, CD13) is a widely expressed, membrane-bound proteolytic enzyme that breaks down peptides during digestion, cleaves cell surface antigens during antigen presentation, and acts as a receptor for human viruses, including several coronaviruses. This multifunctional protein is implicated in the regulation of many biological processes, including angiogenesis, cell proliferation, cell migration, inflammation and immune response (1,2). APN was originally identified as the cell surface antigen CD13, which is expressed in myeloid lineage hematopoietic cells and myeloid leukemia (3). Identified substrates of aminopeptidase N include the angiotensin I-III peptide hormones, the opioid peptide met-enkephalin, and cytokines MCP-1 and MIP-1 (4). Abnormal APN protein expression is seen in various forms of cancer, with high APN expression associated with poor survival in colon cancer and non-small cell lung cancer and silenced APN expression related to poor prognosis in prostate cancer (5-7).

    Alternate Names

    alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; alanyl aminopeptidase, membrane; Aminopeptidase M; Aminopeptidase N; AMPN; ANPEP; AP-M; AP-N; APN; CD13; gp150; hAPN; LAP1; membrane alanyl aminopeptidase; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; P150; PEPN

    For Research Use Only. Not for Use in Diagnostic Procedures.
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