Flow cytometric analysis of live Ramos cells (blue) and Caco-2 cells (green) using CD133 (A8N6N) Mouse mAb (Flow Specific).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Account for isotype matched controls for monoclonal antibodies or species matched IgG for polyclonal antibodies. Count cells using a hemacytometer or alternative method.
posted Februay 2011
Protocol Id: 58
CD133 (A8N6N) Mouse mAb (Flow Specific) recognizes endogenous levels of total CD133 protein.Species Reactivity:
Monoclonal antibody is produced by immunizing animals with cells overexpressing human CD133 protein.
CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicated molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).
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