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51917
CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free) #51917

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using CD133 (D2V8Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from WERI-Rb1 cells, untreated (-) or treated with PNGase F (+), using CD133 (D2V8Q) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using CD133 (D2V8Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD133 (D2V8Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CD133 (D2V8Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human kidney using CD133 (D2V8Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Caco-2 cell pellet (left, positive) or HeLa cell pellet (right, negative) using CD133 (D2V8Q) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of WERI-Rb-1 cells (left, high-expressing) and MCF7 cells (right, low-expressing) using CD133 (D2V8Q) XP® Rabbit mAb (green) and DRAQ5® #4084 (fluorescent DNA dye) (blue). Data were generated using the standard formulation of this product.
To Purchase # 51917
Cat. # Size Qty. Price
51917SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 133
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #64326. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #64326

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD133 (D2V8Q) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD133 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the 1st extracellular loop of human CD133 protein. The epitope has been mapped to a region spanning amino acid residues 303-312. This antibody is not sensitive to the glycosylation status of CD133.

Background

CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).
  1. Yin, A.H. et al. (1997) Blood 90, 5002-12.
  2. Miraglia, S. et al. (1997) Blood 90, 5013-21.
  3. Handgretinger, R. et al. (2003) Ann N Y Acad Sci 996, 141-51.
  4. Monzani, E. et al. (2007) Eur J Cancer 43, 935-46.
  5. O'Brien, C.A. et al. (2007) Nature 445, 106-10.
  6. Ricci-Vitiani, L. et al. (2007) Nature 445, 111-5.
  7. Singh, S.K. et al. (2004) Nature 432, 396-401.
  8. Maw, M.A. et al. (2000) Hum. Mol. Genet. 9, 27-34.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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