CD15/SSEA1 (MC480) Mouse mAb #4744
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | N/A |
| Source/Isotype | Mouse IgM |
Application Key:
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 |
| Flow Cytometry (Live) | 1:1600 |
Storage
For a carrier free (BSA and azide free) version of this product see product #74180.
Protocol
Specificity / Sensitivity
SSEA1 Antibody detects endogenous levels of SSEA1 antigen.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with F9 teratoma cells (X-irradiated).
Background
SSEA1 antibody detects a lactoseries oligosaccharide antigen that is expressed on the surface of mouse embryonal carcinoma and embryonic stem cells (1). This antigen is also found on early mouse embryos and both mouse and human germ cells, but is absent on human embryonic stem and carcinoma cells. Expression of SSEA1 in these human cell types increases upon differentiation, while on the mouse cell types differentiation leads to decreased expression (2).
Limited Uses
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