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Render Timestamp: 2024-10-03T09:49:34.579Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD15/SSEA1 (MC480) Mouse mAb #4744

Filter:
  • IF
  • F

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) N/A
    Source/Isotype Mouse IgM
    Application Key:
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:3200
    Flow Cytometry (Fixed/Permeabilized) 1:1600
    Flow Cytometry (Live) 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #74180.

    Protocol

    Specificity / Sensitivity

    SSEA1 Antibody detects endogenous levels of SSEA1 antigen.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with F9 teratoma cells (X-irradiated).

    Background

    SSEA1 antibody detects a lactoseries oligosaccharide antigen that is expressed on the surface of mouse embryonal carcinoma and embryonic stem cells (1). This antigen is also found on early mouse embryos and both mouse and human germ cells, but is absent on human embryonic stem and carcinoma cells. Expression of SSEA1 in these human cell types increases upon differentiation, while on the mouse cell types differentiation leads to decreased expression (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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