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25121
CD163 (D6U1J) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD163 (D6U1J) Rabbit mAb (BSA and Azide Free) #25121

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  1. WB
  2. IHC
Western Blotting Image 1: CD163 (D6U1J) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from SU-DHL-1 and A-375 cells using CD163 (D6U1J) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD163 (D6U1J) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human serous carcinoma of the ovary using CD163 (D6U1J) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD163 (D6U1J) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD163 (D6U1J) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD163 (D6U1J) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD163 (D6U1J) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD163 (D6U1J) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin lymphoma using CD163 (D6U1J) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CD163 (D6U1J) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded SU-DHL-1 cell pellet (left, positive) or A-375 cell pellet (right, negative) using CD163 (D6U1J) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 25121
Cat. # Size Qty. Price
25121SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 160, 170
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #93498. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #93498.

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD163 (D6U1J) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD163 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the human CD163 protein.

Background

CD163 is a transmembrane scavenger receptor expressed on the macrophage surface. It has 9 B-type SRCR extracellular domains mediating serum haptoglobin clearing/endocytosis, pathogen binding and signal transduction, and calcium binding (1, 2). CD163 is used as a surface marker of M2 type macrophages, including M2 type tumor associated macrophages (TAMs), which facilitate cancer progression by secreting cytokines to promote angiogenesis, immunosuppression and metastasis (3). Inflammatory stimulation and stress signal can induce extracellular domain shedding of CD163 to generate soluble CD163 (sCD163). The increased sCD163 level in serum is associated with low-grade inflammation in disease conditions (4-7).
  1. Graversen, J.H. and Moestrup, S.K. (2015) Membranes (Basel) 5, 228-52.
  2. Etzerodt, A. and Moestrup, S.K. (2013) Antioxid Redox Signal 18, 2352-63.
  3. Komohara, Y. et al. (2014) Cancer Sci 105, 1-8.
  4. Zhi, Y. et al. (2017) Mol Med Rep 15, 2931-2939.
  5. Møller, H.J. et al. (2011) Clin Chem 57, 291-7.
  6. Grønbaek, H. et al. (2012) Aliment Pharmacol Ther 36, 173-80.
  7. Aristoteli, L.P. et al. (2006) Atherosclerosis 184, 342-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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