CD22 (F9D1W) Rabbit mAb #94346
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:50 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Frozen) | 1:1600 - 1:6400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #47369.
Protocol
Specificity / Sensitivity
CD22 (F9D1W) Rabbit mAb recognizes endogenous levels of total CD22 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser467 of mouse CD22 protein.
Background
CD22 (also known as siglec-2) is a member of the sialic acid-binding immunoglobulin-type lectin (Siglec) family of immunomodulatory receptors. CD22 can bind to its ligand α 2,6-linked sialic acid on different cells (trans interaction) as well as on the same cells (cis interaction). CD22 is predominantly expressed on B cells and functions as an inhibitory co-receptor for the B cell receptor (BCR) (1,2). After BCR ligation, the tyrosine kinase Lyn is activated and phosphorylates two distal of the four ITIM motifs in the intracellular carboxy-terminal region of CD22, which then recruit tyrosine phosphatases, including SHP-1, to the plasma membrane, and in turn, they get tyrosine-phosphorylated and activated to damp the signaling pathways initiated by BCR ligation (3,4). CD22 has been actively pursued as a therapeutic target for autoimmune diseases (5). Due to almost exclusive expression on B cells, it is also actively pursued as a therapeutic target for multiple B cell malignancies (6-8).
- Nitschke, L. (2009) Immunol Rev 230, 128-43.
- Meyer, S.J. et al. (2018) Front Immunol 9, 2820.
- Clark, E.A. and Giltiay, N.V. (2018) Front Immunol 9, 2235.
- Enterina, J.R. et al. (2019) Biomed J 42, 218-32.
- Tsubata, T. (2019) Immunol Med 42, 108-16.
- Ai, J. and Advani, A. (2015) Br J Haematol 168, 471-80.
- Yurkiewicz, I.R. et al. (2018) Drug Des Devel Ther 12, 2293-300.
- Pehlivan, K.C. et al. (2018) Curr Hematol Malig Rep 13, 396-406.
Limited Uses
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