CD27 (F3R2V) Rabbit mAb #71135
- IHC
- IF
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:500 - 1:2000 |
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Live) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
CD27 (F3R2V) Rabbit mAb recognizes endogenous levels of total CD27 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse CD27 protein.
Background
CD27 (TNFRSF7) is a transmembrane protein of the TNF receptor superfamily (TNFRSF). It is mainly expressed on lymphoid cells (also on early hematopoietic precursor cells in mice) (1,2). CD27 is considered a phenotypic marker for memory B cells and is also used to identify B regulatory (Breg) cells (3,4). It is constitutively expressed on naïve CD4 and CD8 T cells and its expression is further upregulated upon T cell activation. CD27 is one of the two most important co-stimulatory receptors for T cell priming (the other one is CD28). While CD28 co-stimulatory signal mainly triggers cell proliferation, CD27 co-stimulatory signal primarily promotes cell survival and differentiation (5,6). Upon binding to its ligand CD70, CD27 activates the NF-κB and JNK signaling pathways through TNFR associated factors (TRAFs), the adaptor molecules that are associated with CD27 cytoplasmic tail domain. Upon activation, CD27 is shed from the cell surface and soluble CD27 is used as a marker of T cell activation (7,8).
- So, T. et al. (2006) Int J Hematol 83, 1-11.
- Waight, J.D. et al. (2017) Hum Antibodies 25, 87-109.
- Agematsu, K. (2000) Histol Histopathol 15, 573-6.
- Bouaziz, J.D. et al. (2012) Curr Mol Med 12, 519-27.
- Croft, M. Cytokine Growth Factor Rev 14, 265-73.
- Acuto, O. and Michel, F. (2003) Nat Rev Immunol 3, 939-51.
- Lens, S.M. et al. (1998) Semin Immunol 10, 491-9.
- van de Ven, K. and Borst, J. (2015) Immunotherapy 7, 655-67.
Limited Uses
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