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|3528||CD31 (PECAM-1) (89C2) Mouse mAb||
|77699||CD31 (PECAM-1) (D8V9E) XP® Rabbit mAb||
Flow cytometric analysis of whole blood using CD31 (158-2B3) Mouse mAb.Learn more about how we get our images
Two color flow cytometric analysis of whole blood, using CD31 (1582B3) Mouse mAb and CD5. Pink=granulocytes, blue=monocytes, and green=T cells & B cell subset.Learn more about how we get our images
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation.
NOTE: Count cells using a hemocytometer or alternative method.
posted June 2005
revised June 2017
Protocol Id: 406
Not recommended for use with fixation/permeabilization protocols that contain methanol.
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
CD31 (PECAM-1) (158-2B3) Mouse mAb detects endogenous levels of CD31.
Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leucocytes.
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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