CD36 (D8L9T) Rabbit mAb (BSA and Azide Free) #39914
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70-110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #14347. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
CD36 (D8L9T) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD36 protein. This antibody also cross-reacts with an unidentified protein of 30 kDa.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val112 of human CD36 protein.
Background
CD36 is a class B scavenger receptor composed of short amino-terminal and carboxy-terminal cytoplasmic domains, two transmembrane domains, and a large glycosylated extracellular domain (1-4). The CD36 receptor has many diverse ligands and cellular functions and is expressed by multiple cell types, including monocytes, macrophages, platelets, endothelial cells, adipocytes, and some epithelial cells (1). Binding of thrombospondin-1 (TSP-1) to CD36 facilitates the inhibition of angiogenesis by TSP-1 (5). CD36 also binds lipids and enables their transport into cells (6). In macrophages, CD36 acts as a receptor for oxidized LDL (Ox-LDL) and is responsible for Ox-LDL internalization, which contributes to development of atherosclerosis (7). The CD36 receptor participates in the innate immune response by acting as a pattern recognition receptor for lipid components of bacterial cell walls and fungal beta-glucans (8,9). CD36 likely influences signaling by interacting with other cell surface receptors including TLRs, integrins, and tetraspanins (8,10,11). Phorbol 12-myristate 13-acetate (PMA)/ 12-O-tetradecanoylphorbol-13-acetate (TPA) induces CD36 expression in the THP-1 monocyte cell line (12).
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- Endemann, G. et al. (1993) J Biol Chem 268, 11811-6.
- Dawson, D.W. et al. (1997) J Cell Biol 138, 707-17.
- Kim, Y.R. and Peterkofsky, B. (1997) J Cell Biochem 67, 338-52.
- Nicholson, A.C. et al. (1995) Arterioscler Thromb Vasc Biol 15, 269-75.
- Hoebe, K. et al. (2005) Nature 433, 523-7.
- Means, T.K. et al. (2009) J Exp Med 206, 637-53.
- Mattera, M.D. (1992) RN 55, 7.
- Miao, W.M. et al. (2001) Blood 97, 1689-96.
- Alessio, M. et al. (1996) J Biol Chem 271, 1770-5.
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