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  3. CD4 (Edu-2) Mouse mAb

CD4 (Edu-2) Mouse mAb #3563

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Inquiry Info. # 3563

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 55
    Source/Isotype Mouse IgG2a
    Application Key:
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Not recommended for use with fixation/permeabilization protocols that contain methanol.
    Application Dilution
    Flow Cytometry (Fixed/Permeabilized) 1:25

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD4 (Edu-2) Mouse mAb detects endogenous levels of total CD4 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes.

    Background

    Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part, and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

    Alternate Names

    CD4; CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

    Database Links

    UniProt ID: P01730


    Entrez-Gene Id: 920


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