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CD40 (E2Z7J) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD40 (E2Z7J) Rabbit mAb (BSA and Azide Free) #19554

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  1. WB
  2. IHC
Western Blotting Image 1: CD40 (E2Z7J) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from RAW 264.7 cells, untreated (-) or treated (+) with Lipopolysaccharide #14011 (LPS; 100 ng/ml, 7 hr), using CD40 (E2Z7J) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD40 (E2Z7J) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse adipose tissue using CD40 (E2Z7J) Rabbit mAb. Note staining of lymphoid cells and lack of staining in surrounding white and brown adipose tissues. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD40 (E2Z7J) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse spleen using CD40 (E2Z7J) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD40 (E2Z7J) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded RAW 264.7 cell pellet, untreated (left, negative) or treated with Lipopolysaccharide (LPS) #14011 (middle, positive), and BaF3 cell pellet (right, negative) using CD40 (E2Z7J) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 19554
Cat. # Size Qty. Price
100 µg
$ 746

Supporting Data

MW (kDa) 40
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&T-CUT&Tag
  • DB-Dot Blot
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #86165. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.


Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #86165.


Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD40 (E2Z7J) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total mouse CD40 protein. This antibody does not cross-react with human CD40 protein.

Species Reactivity:


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val289 of mouse CD40 protein.


CD40, also known as tumor necrosis factor receptor superfamily member 5 (TNFRSF5), is a type I transmembrane protein expressed on the surface of B cells and professional antigen-presenting cells of the immune system, as well as on several non-hematopoietic cell types and cancers (1-4). CD40 interacts with CD40 ligand (CD40L/TNFSF5), which is expressed primarily on activated T cells but has also been reported on blood platelets, mast cells, basophils, NK cells, and B cells (5). Upon engagement with CD40L, CD40 signals through TNF receptor associated factors and MAP kinase signaling pathways, resulting in a wide variety of immune and inflammatory responses, including dendritic cell activation and cross-presentation, T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation (6-8). The CD40/CD40L axis is essential for the initiation and progression of cellular and humoral adaptive immunity, and is an important area of interest in the study of tumor immunology, neurodegenerative diseases, vascular diseases, and inflammatory disorders (9-12).
  1. Banchereau, J. et al. (1995) Adv Exp Med Biol 378, 79-83.
  2. Cooke, P.W. et al. (1999) J Pathol 188, 38-43.
  3. Hakkarainen, T. et al. (2003) Clin Cancer Res 9, 619-24.
  4. Slobodova, Z. et al. (2011) Neoplasma 58, 189-97.
  5. Gauchat, J.F. et al. (1993) FEBS Lett 315, 259-66.
  6. Quezada, S.A. et al. (2004) Annu Rev Immunol 22, 307-28.
  7. Spriggs, M.K. et al. (1992) J Exp Med 176, 1543-50.
  8. Elgueta, R. et al. (2009) Immunol Rev 229, 152-72.
  9. Korniluk, A. et al. (2014) Tumour Biol 35, 9447-57.
  10. Townsend, K.P. et al. (2005) Eur J Immunol 35, 901-10.
  11. Hassan, G.S. et al. (2012) Immunobiology 217, 521-32.
  12. Zhang, B. et al. (2013) Immunol Lett 153, 58-61.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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