Interested in promotions? | Click here >>
97253
CD47 (D3O7P) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD47 (D3O7P) Rabbit mAb (BSA and Azide Free) #97253

Reviews ()
Citations (0)
Filter:
  1. WB
  2. IHC
Western Blotting Image 1: CD47 (D3O7P) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using CD47 (D3O7P) Rabbit mAb (upper) or COX IV (3E11) Rabbit mAb #4850 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD47 (D3O7P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human ovarian serous carcinoma using CD47 (D3O7P) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD47 (D3O7P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CD47 (D3O7P) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD47 (D3O7P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CD47 (D3O7P) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD47 (D3O7P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CD47 (D3O7P) Rabbit mAb (left) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CD47 (D3O7P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded OVCAR3 cell pellet (left, positive) or Hep G2 cell pellet (right, negative) using CD47 (D3O7P) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 97253
Cat. # Size Qty. Price
97253SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 45-50
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #63000. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #63000

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CD47 (D3O7P) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD47 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu72 of human CD47 protein.

Background

CD47 is a five-pass transmembrane protein expressed on all normal cells. It binds to the SIRPa that is expressed on myeloid cells including macrophages, and neuronal cells in the central nervous system. Binding of CD47 to SIRPα promotes phosphorylation of tyrosine residues in the immunoreceptor tyrosine-based inhibitory motifs (ITIM) within theSIRPα cytoplasmic tail, inhibiting macrophage phagocytosis towards CD47-expressing cells. In this way, CD47 serves as "don't eat me" signal or a marker of "self", functioning as an innate immune checkpoint. Additionally, CD47 was reported to modulate lymphocyte cell activation and proliferation (1-3). CD47 is over-expressed in many types of cancer. The expression level of CD47 on cancer cells is negatively associated with the response to therapies, and low expression on tumor cells is associated with a better prognosis and survival. Reagents that can block CD47-SIRPα interaction are being actively pursued for therapeutic applications (4,5). In addition to SIRPα, other proteins have been reported to bind to CD47. Thrombospondin 1 (TSP1) competes with SIRPα to bind to CD47 in the extracellular region and activates signaling pathways downstream CD47 (6). CD47 can laterally associate with VEGFR2, FAS, and certain integrins in different contexts, and influences their downstream signaling (7-9). CD47 can be shed from the cell surface by proteolytic cleavage. In addition, CD47 is present on extracellular vesicles including exosomes, suggesting additional extracellular signaling potential (10).
  1. Murata, Y. et al. (2014) J Biochem 155, 335-44.
  2. Legrand, N. et al. (2011) Proc Natl Acad Sci U S A 108, 13224-9.
  3. Barclay, A.N. and Van den Berg, T.K. (2014) Annu Rev Immunol 32, 25-50.
  4. Weiskopf, K. (2017) Eur J Cancer 76, 100-109.
  5. Matlung, H.L. et al. (2017) Immunol Rev 276, 145-164.
  6. Roberts, D.D. et al. (2012) Matrix Biol 31, 162-9.
  7. Kaur, S. et al. (2010) J Biol Chem 285, 38923-32.
  8. Azcutia, V. et al. (2013) Mol Biol Cell 24, 3358-68.
  9. Quesada, A.J. et al. (2005) Cell Death Differ 12, 649-58.
  10. Soto-Pantoja, D.R. et al. (2015) Crit Rev Biochem Mol Biol 50, 212-30.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.