CD5 (E5O4W) Rabbit mAb #28294
- IF
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:800 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
CD5 (E5O4W) Rabbit mAb recognizes endogenous levels of total CD5 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala219 of mouse CD5 protein.
Background
CD5 is a type-I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) family, characterized by the presence of at least one SRCR domain of 90-110 amino acids. CD5 is expressed by all mature T cells, the B-1a subset of mature B cells, and some leukemic B cells. Its expression is increased in regulatory T and B cells (Tregs/Bregs). Anergic T and B cells also have elevated CD5 expression. Elevated levels of CD5 are also found in many autoimmune disorders (1-3). CD5 is associated with the T cell receptor (TCR) and negatively modulates T cell activation and differentiation. CD5 expression on the tumor infiltrating T lymphocytes is inversely correlated with their antitumor activity (4-6). Recently, it was reported that CD5 directly binds to IL6 and can mediate downstream signaling. CD5+ B cells promote tumor growth in animal models (7). Reagents targeting CD5 have been actively pursued as therapeutic interventions for cancer and other conditions (8,9).
- Raman, C. (2002) Immunol Res 26, 255-63.
- Soldevila, G. et al. (2011) Curr Opin Immunol 23, 310-8.
- Sigal, L.H. (2012) J Clin Rheumatol 18, 83-8.
- Azzam, H.S. et al. (2001) J Immunol 166, 5464-72.
- Dalloul, A. (2009) Autoimmun Rev 8, 349-53.
- Henderson, J.G. et al. (2015) Immunity 42, 471-83.
- Zhang, C. et al. (2016) Immunity 44, 913-923.
- Consuegra-Fernández, M. et al. (2015) Crit Rev Immunol 35, 85-115.
- Vasquez, M. et al. (2017) Eur J Immunol 47, 1108-1118.
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