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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD58 (F7R6O) Rabbit mAb #63246

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  • WB
  • IP
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 55-70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400
    Flow Cytometry (Live) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD58 (F7R6O) Rabbit mAb recognizes endogenous levels of total CD58 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD58 protein.

    Background

    CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a ubiquitously expressed glycoprotein anchored to the plasma membrane through a transmembrane domain or a GPI linkage (1). Research studies have demonstrated that CD58 plays a critical role in immune synapse formation by functioning as a co-stimulatory adhesion molecule upon engagement of the CD2 antigen on the surface of T cells and NK cells. As such, the CD58-CD2 axis facilitates the execution of immune effector cell functions, including activation, proliferation, and cytotoxicity (2-4). In the context of cancer, recent studies have pointed to the CD58-CD2 axis as a stimulatory immune checkpoint, and lesions to this axis compromise anti-tumor immune responses (5,6).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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