CD59 (E7F7G) Rabbit mAb #25393
- WB
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Live) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
CD59 (E7F7G) Rabbit mAb recognizes endogenous levels of total CD59 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu79 of human CD59 protein.
Background
CD59 is a GPI-anchored membrane protein that functions as an inhibitor of the complement membrane attack complex (MAC). CD59 binds to complement components C8 and C9, preventing C9 polymerization and insertion into membranes, therefore inhibiting the complement-dependent cytolysis (CDC) (1). CD59 is a ubiquitously expressed cell membrane protein that protects cells from CDC. Rare cases of CD59 deficiency have been reported to cause paroxysmal nocturnal hemoglobinuria in human patients (2,3). Expression of CD59 on tumor cells and viral infected cells makes them resist antibody-dependent complement-mediated lysis. Potent inhibitors for CD59 have been actively pursued for therapeutic applications (4,5). In addition, CD59 may regulate insulin secretion by modulating exocytosis (6).
- Sugita, Y. and Masuho, Y. (1995) Immunotechnology 1, 157-68.
- Mevorach, D. (2015) Mol Immunol 67, 51-5.
- Höchsmann, B. and Schrezenmeier, H. (2015) Hematol Oncol Clin North Am 29, 495-507.
- Cai, B. et al. (2014) J Biol Chem 289, 12109-25.
- Lan, J. et al. (2014) J Immunol 193, 3577-89.
- Krus, U. et al. (2014) Cell Metab 19, 883-90.
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