CD6 (E9Y7Y) Rabbit mAb (BSA and Azide Free) #36549
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100-140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
CD6 (E9Y7Y) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD6 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD6 protein.
Background
T cell differentiation antigen CD6 is a cell adhesion molecule expressed on immature thymocytes and mature T cells, and has also been detected on a subset of B cells and NK cells within the immune system (1-4). CD6 mediates cell-cell interactions through its binding partner CD166/ALCAM (2), and contributes to the formation and maturation of the immunological synapse (3,4). CD6 functions as a co-stimulatory receptor, promoting T cell activation and proliferation through the TCR/CD3 complex signaling cascade (3-6). Studies have shown CD6 can be glycosylated (7), hyperphosphorylated on serine and threonine residues (8), and phosphorylated on tyrosine residues (6,9), each of which can differentially affect the function and signaling of this molecule. CD6 also functions as a calcium-dependent pattern receptor that binds and aggregates Gram-positive and Gram-negative bacteria. In response to lipopolysaccharide, CD6 mediates activation of the inflammatory response and secretion of pro-inflammatory cytokines (10).
- Bowen, M.A. et al. (1997) J Immunol 158, 1149-56.
- Hassan, N.J. et al. (2004) Eur J Immunol 34, 930-40.
- Gimferrer, I. et al. (2004) J Immunol 173, 2262-70.
- Zimmerman, A.W. et al. (2006) Blood 107, 3212-20.
- Roncagalli, R. et al. (2014) Nat Immunol 15, 384-392.
- Hassan, N.J. et al. (2006) Mol Cell Biol 26, 6727-38.
- Escoda-Ferran, C. et al. (2014) FEBS Lett 588, 2805-13.
- Swack, J.A. et al. (1991) J Biol Chem 266, 7137-43.
- Wee, S. et al. (1993) J Exp Med 177, 219-23.
- Sarrias, M.R. et al. (2007) Proc Natl Acad Sci USA 104, 11724-9.
Limited Uses
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