CD63 (F8P5K) Rabbit mAb #10112
- WB
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
CD63 (F8P5K) Rabbit mAb recognizes endogenous levels of total CD63 protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn150 of mouse CD63 protein.
Background
CD63 belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2) (1-3). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin-enriched microdomains (TEMs) where they mediate a range of processes, including adhesion, motility, membrane organization, and signal transduction (3). CD63, like other tetraspanins, is enriched in exosomes (4). It is also a component of Weibel-Palade bodies found in endothelial cells (5). Research studies demonstrate several functions of CD63 in different cell types, including roles in mast cell degranulation, VEGF signaling in endothelial cells, recruitment of leukocytes to endothelial cells, and endosomal sorting during melanogenesis (6-9).
- Oren, R. et al. (1990) Mol Cell Biol 10, 4007-15.
- Levy, S. et al. (1991) J Biol Chem 266, 14597-602.
- Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
- Escola, J.M. et al. (1998) J Biol Chem 273, 20121-7.
- Vischer, U.M. and Wagner, D.D. (1993) Blood 82, 1184-91.
- Kraft, S. et al. (2013) J Immunol 191, 2871-8.
- Tugues, S. et al. (2013) J Biol Chem 288, 19060-71.
- Doyle, E.L. et al. (2011) Blood 118, 4265-73.
- van Niel, G. et al. (2011) Dev Cell 21, 708-21.
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